Isoliquiritigen enhances the antitumour activity and decreases the genotoxic effect of cyclophosphamide.


The aim of this study was to evaluate the antitumour activities and genotoxic effects of isoliquiritigenin (ISL) combined with cyclophosphamide (CP) in vitro and in vivo. U14 cells were treated with either of ISL (5-25 μg/mL) or CP (0.25-1.25 mg/mL) alone or with combination of ISL (5-25 μg/mL) and CP (1.0 mg/mL) for 48 h. The proliferation inhibitory effect in vitro was evaluated by MTT and colony formation assays. KM mice bearing U14 mouse cervical cancer cells were used to estimate the antitumour activity in vivo. The genotoxic activity in bone marrow polychromatic erythrocytes was assayed by frequency of micronuclei. The DNA damage in peripheral white blood cells was assayed by single cell gel electrophoresis. The results showed that ISL enhanced antitumour activity of CP in vitro and in vivo, and decreased the micronucleus formation in polychromatic erythrocytes and DNA strand breaks in white blood cells in a dose-dependent way.

DOI: 10.3390/molecules18088786

Extracted Key Phrases

6 Figures and Tables

Citations per Year

Citation Velocity: 8

Averaging 8 citations per year over the last 3 years.

Learn more about how we calculate this metric in our FAQ.

Cite this paper

@article{Zhao2013IsoliquiritigenET, title={Isoliquiritigen enhances the antitumour activity and decreases the genotoxic effect of cyclophosphamide.}, author={Hong Zhao and Xuan Yuan and Defang Li and Hongmei Chen and Jiangtao Jiang and Zhiping Wang and Xiling Sun and Qiusheng Zheng}, journal={Molecules}, year={2013}, volume={18 8}, pages={8786-98} }