Isolation of virulence genes directing GPI synthesis by functional complementation of Leishmania.

@article{Turco1994IsolationOV,
  title={Isolation of virulence genes directing GPI synthesis by functional complementation of Leishmania.},
  author={Salvatore Joseph Turco and Albert Descoteaux and Kathleen A. Budziszewski Ryan and Levi A. Garraway and Stephen M Beverley},
  journal={Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas},
  year={1994},
  volume={27 2},
  pages={
          133-8
        }
}
Recent advances in molecular genetics of Leishmania parasites prompted us to develop methods of functional genetic complementation in Leishmania and apply them to the isolation of genes involved in the biosynthesis of the virulence determinant LPG, an abundant GPI-anchored polysaccharide. LPG1, the gene product identified by complementation of our R2D2 LPG- mutant, may be a glycosyltransferase responsible for the addition of galactofuranose to the nascent chain. As galactofuranose is not found… CONTINUE READING