Isolation of two distinct populations of cells from rat kidney cortex and their use in the study of chemical-induced toxicity.

@article{Lash1989IsolationOT,
  title={Isolation of two distinct populations of cells from rat kidney cortex and their use in the study of chemical-induced toxicity.},
  author={Lawrence H Lash and J J Tokarz},
  journal={Analytical biochemistry},
  year={1989},
  volume={182 2},
  pages={
          271-9
        }
}
Procedures for the isolation and enrichment of cell populations from suspensions of rat kidney cortical cells were developed. Using Percoll density-gradient centrifugation, two populations of cells were obtained; marker enzymes [alkaline phosphatase and gamma-glutamyltransferase for proximal tubular (PT) cells and hexokinase for distal tubular (DT) cells] and functional responses (stimulation of PT cell oxygen consumption by succinate and inhibition of DT cell oxygen consumption by amiloride… CONTINUE READING

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