Isolation of two beta-xylosidase genes of Bacillus pumilus and comparison of their gene products.

@article{Panbangred1984IsolationOT,
  title={Isolation of two beta-xylosidase genes of Bacillus pumilus and comparison of their gene products.},
  author={Watanalai Panbangred and Osamu Kawaguchi and Takashi Tomita and Atsuhiko Shinmyō and Hirosuke Okada},
  journal={European journal of biochemistry},
  year={1984},
  volume={138 2},
  pages={267-73}
}
The chromosomal DNA fragments of Bacillus pumilus IPO, a potent xylan-hydrolyzing bacterium, were ligated to a vector plasmid, pBR322, and used to transform Escherichia coli C600 cells. Two hybrid plasmids, pOXD28 and pOXN29, were found to enable the transformants to produce beta-xylosidase. The former was found to contain a 2.6-MDa Bg/II fragment and the… CONTINUE READING