Isolation of tumor-secreted products from human carcinoma cells maintained in a defined protein-free medium.

@article{Alderman1985IsolationOT,
  title={Isolation of tumor-secreted products from human carcinoma cells maintained in a defined protein-free medium.},
  author={E M Alderman and Roy R. Lobb and James W. Fett},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={1985},
  volume={82 17},
  pages={5771-5}
}
A protein-free synthetic cell-growth medium has been defined that permits long-term survival (greater than 120 days) of an established human colon tumor cell line, HT-29. Viability is dependent upon both the concentration of L-glutamine in the medium and the cell density at the time of initial transfer into it. Cell proliferation is minimal, thus obviating the necessity for subculturing. HT-29 adenocarcinoma cells maintained in large-scale culture with this medium continue to secrete the… CONTINUE READING