Isolation of transposon Tn551 insertions near chromosomal markers of interest in Staphylococcus aureus.

@article{Luchansky1984IsolationOT,
  title={Isolation of transposon Tn551 insertions near chromosomal markers of interest in Staphylococcus aureus.},
  author={John B. Luchansky and P. A. Pattee},
  journal={Journal of bacteriology},
  year={1984},
  volume={159 3},
  pages={894-9}
}
A procedure was developed to isolate insertions of transposon Tn551 near other markers of interest on the chromosome of Staphylococcus aureus NCTC 8325. When an inoculum of strain 8325-4 carrying a thermosensitive mutant of plasmid pI258 (on which Tn551 resides) was inoculated into brain heart infusion agar plus erythromycin and grown to saturation at 43 degrees C, the transforming DNA extracted from this population of cells contained a random collection of different chromosomal insertions of… CONTINUE READING
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Erythromycin-sensitive mutations of transposon Tn551 in Staphylococcus aureus

  • P. A. Pattee, C. J. Schroeder, M. L. Stahl
  • Iowa State J. Res
  • 1983
1 Excerpt

Transduction analysis of transposon Tn 551 insertions in the trp - thy region of the Staphylococcus aureus chromosome

  • P. A. Pattee
  • J . Bacteriol .
  • 1983

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