Isolation of transporting plasma membrane vesicles from bovine tracheal epithelium.

@article{LangridgeSmith1983IsolationOT,
  title={Isolation of transporting plasma membrane vesicles from bovine tracheal epithelium.},
  author={Jane E. Langridge-Smith and Michael H. Field and William P. Dubinsky},
  journal={Biochimica et biophysica acta},
  year={1983},
  volume={731 2},
  pages={318-28}
}
A method is described for isolating plasma membrane vesicles from bovine tracheal epithelium. The procedure yields highly purified apical membranes which are enriched 19-fold in the marker enzyme, alkaline phosphatase. Contamination of this fraction by other organelles is minimal. Basolateral membranes isolated from the same preparation have a 4-fold enrichment of (Na+ + K+)-ATPase and a 2-fold reduction in alkaline phosphatase specific activity compared to the starting material. Assays of Na… CONTINUE READING

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