Isolation of the yeast nuclear pore complex

@article{Rout1993IsolationOT,
  title={Isolation of the yeast nuclear pore complex},
  author={Michael P. Rout and G{\"u}nter Blobel},
  journal={The Journal of Cell Biology},
  year={1993},
  volume={123},
  pages={771 - 783}
}
Nuclear pore complexes (NPCs) have been isolated from the yeast Saccharomyces. Negative stain electron microscopy of the isolated NPCs and subsequent image reconstruction revealed the octagonal symmetry and many of the ultrastructural features characteristic of vertebrate NPCs. The overall dimensions of the yeast NPC, both in its isolated form as well as in situ, are smaller than its vertebrate counterpart. However, the diameter of the central structures are similar. The isolated yeast NPC has… CONTINUE READING
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The three dimensional structure of the nuclear pore complex as seen by high voltage electron microscopy and high resolution low voltage scanning electron microscopy

H. Ris
EMSA Bulletin. 21:54-56. • 1991
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