Isolation of the yeast histone octamer.

  title={Isolation of the yeast histone octamer.},
  author={Yahli Lorch and Roger D. Kornberg},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  volume={91 23},
Procedures for the extraction and purification of the yeast histone octamer are described. Either mechanical disruption, yielding chromatin fragments, or spheroplast formation with subsequent nuclear isolation was employed. A hexahistidine tag was inserted in the N-terminal region of histone H2B, permitting resolution of the histone octamer from high-salt extracts of nuclei or chromatin to near homogeneity. The histone octamer purified in this way was fully active in reconstitution of… CONTINUE READING