Isolation of the ryanodine receptor from cardiac sarcoplasmic reticulum and identity with the feet structures.

@article{Inui1987IsolationOT,
  title={Isolation of the ryanodine receptor from cardiac sarcoplasmic reticulum and identity with the feet structures.},
  author={Makoto Inui and Akitsugu Saito and Sidney Fleischer},
  journal={The Journal of biological chemistry},
  year={1987},
  volume={262 32},
  pages={15637-42}
}
Ryanodine, a highly toxic alkaloid, reacts specifically with the Ca2+ release channels which are localized in the terminal cisternae of sarcoplasmic reticulum (SR). In this study, the ryanodine receptor from cardiac SR has been purified, characterized, and compared with that of skeletal muscle SR. The ryanodine receptor was solubilized with 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate (CHAPS) in the presence of phospholipids. Purification was performed by sequential affinity… CONTINUE READING

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