Isolation of the repertoire of VSG expression site containing telomeres of Trypanosoma brucei 427 using transformation-associated recombination in yeast.

@article{Becker2004IsolationOT,
  title={Isolation of the repertoire of VSG expression site containing telomeres of Trypanosoma brucei 427 using transformation-associated recombination in yeast.},
  author={Marion M. Becker and Niall Aitcheson and Elaine D Byles and Bill Wickstead and Edward J Louis and Gloria Rudenko},
  journal={Genome research},
  year={2004},
  volume={14 11},
  pages={2319-29}
}
Trypanosoma brucei switches between variant surface glycoproteins (VSGs) allowing immune escape. The active VSG is in one of many telomeric bloodstream form VSG expression sites (BESs), also containing expression site-associated genes (ESAGs) involved in host adaptation. The role of BES sequence diversity in parasite virulence can best be understood through analysis of the full repertoire of BESs from a given T. brucei strain. However, few BESs have been cloned, as telomeres are highly… CONTINUE READING