Isolation of the pharmacologically active saponin ginsenoside Rb1 from ginseng by immunoaffinity column chromatography.

@article{Fukuda2000IsolationOT,
  title={Isolation of the pharmacologically active saponin ginsenoside Rb1 from ginseng by immunoaffinity column chromatography.},
  author={Naoya Fukuda and Hiroyuki Tanaka and Yukihiro Shoyama},
  journal={Journal of natural products},
  year={2000},
  volume={63 2},
  pages={283-5}
}
Immunoaffinity column chromatography using an anti-ginsenoside Rb1 monoclonal antibody has made possible a single-step separation of ginsenoside Rb1 from a crude extract of ginseng roots (Panax ginseng). The combination of immunoaffinity column chromatography and an enzyme-linked immunosorbent assay (ELISA) was also investigated.