Isolation of the nitrogen assimilation regulator NR(I), the product of the glnG gene of Escherichia coli.

@article{Reitzer1983IsolationOT,
  title={Isolation of the nitrogen assimilation regulator NR(I), the product of the glnG gene of Escherichia coli.},
  author={Larry Reitzer and Boris Magasanik},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={1983},
  volume={80 18},
  pages={5554-8}
}
The product of the glnG gene, a member of the complex glnALG operon, is an essential component in the response of Escherichia coli K-12 and other enteric bacteria to nitrogen-limited growth. We have purified this protein which we propose to call "NR(I)," for nitrogen regulator I, to about 95% purity from an overproducing strain. Purified NR(I) was identified as a dimer by gel filtration. NR(I) specifically inhibited initiation of transcription from a DNA fragment containing the glnL promoter… CONTINUE READING

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