Isolation of the galactose-binding lectin that mediates the in vitro adherence of Entamoeba histolytica.

@article{Petri1987IsolationOT,
  title={Isolation of the galactose-binding lectin that mediates the in vitro adherence of Entamoeba histolytica.},
  author={William A Petri and Reginald D Smith and Paul H Schlesinger and Christina Murphy and Jonathan I Ravdin},
  journal={The Journal of clinical investigation},
  year={1987},
  volume={80 5},
  pages={1238-44}
}
Entamoeba histolytica adheres to human colonic mucus, colonic epithelial cells, and other target cells via a galactose (Gal) or N-acetyl-D-galactosamine (GalNAc) inhibitable surface lectin. Blockade of this adherence lectin with Gal or GalNAc in vitro prevents amebic killing of target cells. We have identified and purified the adherence lectin by two methods: affinity columns derivatized with galactose monomers or galactose terminal glycoproteins, and affinity columns and immunoblots prepared… CONTINUE READING