Isolation of the (+)-Pinoresinol-Mineralizing Pseudomonas sp. Strain SG-MS2 and Elucidation of Its Catabolic Pathway

  title={Isolation of the (+)-Pinoresinol-Mineralizing Pseudomonas sp. Strain SG-MS2 and Elucidation of Its Catabolic Pathway},
  author={Madhura Shettigar and Sahil Balotra and David M. Cahill and Andrew C. Warden and Michael James Lacey and Hans-Peter E. Kohler and Daniel Rentsch and John G. Oakeshott and Gunjan Pandey},
  journal={Applied and Environmental Microbiology},
ABSTRACT Pinoresinol is a dimer of two β-β′-linked coniferyl alcohol molecules. It is both a plant defense molecule synthesized through the shikimic acid pathway and a representative of several β-β-linked dimers produced during the microbial degradation of lignin in dead plant material. Until now, little has been known about the bacterial catabolism of such dimers. Here we report the isolation of the efficient (+)-pinoresinol-mineralizing Pseudomonas sp. strain SG-MS2 and its catabolic pathway… 
7 Citations
Oxidative Catabolism of (+)-Pinoresinol Is Initiated by an Unusual Flavocytochrome Encoded by Translationally Coupled Genes within a Cluster of (+)-Pinoresinol-Coinduced Genes in Pseudomonas sp. Strain SG-MS2
Comp comparative genomics, proteomics, protein semipurification, and heterologous expression are used to identify a flavoprotein from the vanillyl alcohol oxidase/p-cresol methyl hydroxylase (VAO/PCMH) enzyme family in SG-MS2 that carries out the initial hydroxymation of (+)-pinoresinol at the benzylic carbon.
The Hydroxyquinol Degradation Pathway in Rhodococcus jostii RHA1 and Agrobacterium Species Is an Alternative Pathway for Degradation of Protocatechuic Acid and Lignin Fragments
This study has found the existence of a second pathway for degradation of protocatechuic acid in Rhodococcus jostii RHA1, via hydroxyquinol (benzene-1,2,4-triol), which establishes a metabolic link between protocatechuate 3, 4-dioxygenase and hydroxychinol.
Characterization of Thiamine Diphosphate-Dependent 4-Hydroxybenzoylformate Decarboxylase Enzymes from Rhodococcus jostii RHA1 and Pseudomonas fluorescens Pf-5 Involved in Degradation of Aryl C2 Lignin Degradation Fragments.
The P. fluorescens decarboxylase enzyme showed enhanced carboligase activity between vanillin and acetaldehyde, rationalized by the presence of alanine versus serine at residue 73 in the enzyme active site, which was investigated further by site-directed mutagenesis of this residue.
Mapping the diversity of microbial lignin catabolism: experiences from the eLignin database
The eLignin database is introduced, its dataset is used to map the reported ecological and biochemical diversity of the lignin microbial niches, and the findings are discussed.
Vanillyl alcohol oxidase.
This review presents a historical outline of the research on vanillyl alcohol oxidase (VAO) from Penicillium simplicissimum, one of the canonical members of the VAO/PCMH flavoprotein family, and discusses the physiological role, substrate scope, and catalytic mechanism of VAO.
Advances in microbial lignin degradation and its applications.
This review focuses on microbial enzymes that are responsible for lignin degradation and on their applications to biological lignocellulosic biomass pretreatment and biotechnological lign in engineering.
Potential use of bacteria collected from human hands for textile dye decolorization
Abstract We obtained dye-decolorizing bacteria from the hands of ordinary people with an efficiency of approximately 40%. The bacteria were classified into the azo dye-decolorizing group and


Discovery of pinoresinol reductase genes in sphingomonads.
Bacterial genes for the degradation of major dilignols produced in lignifying xylem are expected to be useful tools for the structural modification of lignin in plants. For this purpose, we isolated
Phylogenetic and Kinetic Characterization of a Suite of Dehydrogenases from a Newly Isolated Bacterium, Strain SG61-1L, That Catalyze the Turnover of Guaiacylglycerol-β-Guaiacyl Ether Stereoisomers
A suite of dehydrogenase candidates were heterologously expressed and kinetically characterized and exhibited stereoselectivity toward the GGE stereoisomers, indicating a variety of potential applications for these enzymes in the manufacture of lignin-derived commodities.
(+)-Pinoresinol/(+)-Lariciresinol Reductase from Forsythia intermedia
Lignans are a widely distributed class of natural products, whose functions and distribution suggest that they are one of the earliest forms of defense to have evolved in vascular plants; some, such
Cloning and Biochemical Characterization of a Novel Carbendazim (Methyl-1H-Benzimidazol-2-ylcarbamate)-Hydrolyzing Esterase from the Newly Isolated Nocardioides sp. Strain SG-4G and Its Potential for Use in Enzymatic Bioremediation
A highly efficient carbendazim (methyl-1H-benzimidazol-2-ylcarbamate, or MBC)-mineralizing bacterium was isolated from enrichment cultures originating from MBC-contaminated soil samples, and the initial steps of this novel metabolic pathway were confirmed by growth and enzyme assays and liquid chromatography-mass spectrometry studies.
Genetic and Biochemical Investigations on Bacterial Catabolic Pathways for Lignin-Derived Aromatic Compounds
The enzyme systems for β-aryl ether and biphenyl degradation, O demethylation linked with one carbon metabolism, the PCA 4,5-cleavage pathway, and the multiple 3MGA catabolic pathways in SYK-6 are outlined.
Degradation of d,l-syringaresinol, a β-β′ linked lignin model compound, by Fusarium solani M-13-1
A β-β′ linked lignin model compound, d,l-syringaresinol monobenzyl ether (Ib) was incubated with Fusarium solani M-13-1 in a shaking culture. From the culture filtrates, three compounds II, IIIb and
Degradation of Trimeric Lignin Model Compounds, Arylglycerol-β-syringaresinol Ethers, by Fusarium solani M-13-1
Trimeric lignin model compounds, arylglycerol-β-syringaresinol ethers, were incubated with Fusarium solani M-13-1 in basal salts medium with shaking. From the culture filtrates, degradation products
Characterization of Arabidopsis thaliana Pinoresinol Reductase, a New Type of Enzyme Involved in Lignan Biosynthesis*
It was demonstrated conclusively for the first time that differential expression of PrR isoforms that have distinct selectivities of substrate enantiomers can determine enantiomeric compositions of the product, lariciresinol.
Initial Steps in the Pathway for Bacterial Degradation of Two Tetrameric Lignin Model Compounds
The metabolic route by which a lignin tetramer-degrading mixed bacterial culture degraded two tetrameric lign in model compounds containing beta-O-4 and 5-5 biphenyl structures was different from the route of the mainligninase-catalyzed C(alpha)-C(beta) cleavage by Phanerochaete chrysosporium.
Hydroquinone as the Ring-fission Substrate in the Catabolism of 4-Ethylphenol and 4-Hydroxyacetophenone by Pseudomonas putida JD1
SUMMARY: A bacterium capable of growth on 4-ethylphenol was isolated from soil and identified as Pseudomonas putida. Intact cells grown on 4-ethylphenol rapidly oxidized 4-hydroxyaceto-phenone as