Isolation of temperature sensitive mutants and their mapping in Agrobacterium tumefaciens.

@article{Taga2002IsolationOT,
  title={Isolation of temperature sensitive mutants and their mapping in Agrobacterium tumefaciens.},
  author={Tomoko Taga and Masami Hino and Katsunori Suzuki and Kazuo Yoshida},
  journal={Nucleic acids research. Supplement},
  year={2002},
  volume={2},
  pages={
          89-90
        }
}
The first temperature sensitive mutants of plant pathogenic bacterium Agrobacterium tumefaciens were isolated by Tn5 mutagenesis. Several genes responsible for mutants were identified and mapped on the circular and linear chromosomes in A. tumefaciens MAFF301001. 

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