Isolation of suppressors of temperature-sensitive folding mutations.

  title={Isolation of suppressors of temperature-sensitive folding mutations.},
  author={R. Villafane and A. Fleming and C. Haase-Pettingell},
  journal={Journal of bacteriology},
  volume={176 1},
Mutations in the tailspike gene (gene 9) of Salmonella typhimurium phage P22 have been used to identify amino acid interactions during the folding of a polypeptide chain. Since temperature-sensitive folding (tsf) mutations cause folding defects in the P22 tailspike polypeptide chain, it is likely that mutants derived from these and correcting the original tsf defects (second-site intragenic suppressors) identify interactions during the folding pathway. We report the isolation and identification… Expand
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