Isolation of subcellular fractions from the yeast Saccharomyces cerevisiae.

  title={Isolation of subcellular fractions from the yeast Saccharomyces cerevisiae.},
  author={Stephanie E. Rieder and Scott D Emr},
  journal={Current protocols in cell biology},
  volume={Chapter 3},
  pages={Unit 3.8}
This unit presents detailed protocols for a range of centrifugation-based subcellular fractionation procedures for the yeast Saccharomyces cerevisiae. Techniques include spheroplast preparation, glass-bead lysis, differential centrifugation, and several density gradient procedures using a variety of gradient media. There are analytical procedures that are primarily designed to evaluate the association of proteins with organelles in the exocytic and endocytic pathways. Additionally, there are… CONTINUE READING

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See above. This review provides an excellent summary of the subcellular fractionation techniques applied to S. cerevisiae and the corresponding references. Internet Resources

  • Zinser, Daum
  • 1995
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