Isolation of strong expression signals of Mycobacterium tuberculosis.

@article{Triccas2001IsolationOS,
  title={Isolation of strong expression signals of Mycobacterium tuberculosis.},
  author={James A Triccas and Warwick John Britton and B. Gicquel},
  journal={Microbiology},
  year={2001},
  volume={147 Pt 5},
  pages={1253-8}
}
The natural fluorescence of the Aequoria victoria green fluorescent protein was exploited to isolate strong expression signals of Mycobacterium tuberculosis. Mycobacterium bovis bacille Calmette-Guérin harbouring M. tuberculosis fragments driving high levels of gfp expression were isolated by fluorescence-activated cell sorting (FACS). DNA sequencing and subsequent comparison with the M. tuberculosis genome sequence revealed that a total of nine postulated promoters had been identified. The… CONTINUE READING
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