Isolation of spontaneously derived mutants of Caulobacter crescentus.

  title={Isolation of spontaneously derived mutants of Caulobacter crescentus.},
  author={Reid C Johnson and Bert Ely Ely},
  volume={86 1},
Caulobacter crescentus has a penicillinase which precludes the use of penicillin for mutant enrichment. However, two other antibiotics, fosfomycin and D-cycloserine, can be enrich for C. crescentus mutants. In enrichment procedures for C. crescentus auxotrophs, spontaneously derived mutants occur at a frequency of 5-10% among the survivors of an enrichment procedure. Consequently, large numbers of mutants are readily obtained without any need for mutagenesis. These mutants are heterogeneous… CONTINUE READING

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