Isolation of sinusoidal and canalicular liver plasma membranes: Effects of frozen storage of human material.

@article{Edwards1994IsolationOS,
  title={Isolation of sinusoidal and canalicular liver plasma membranes: Effects of frozen storage of human material.},
  author={Robert J. Edwards and Cynthia L Leslie and Neill H. Stacey},
  journal={Toxicology in vitro : an international journal published in association with BIBRA},
  year={1994},
  volume={8 2},
  pages={173-80}
}
Sinudoisal and canalicular plasma membranes were prepared from rat and fresh or frozen human liver and characterized using plasma membrane marker enzymes. The distributions of these enzymes were used as a means of comparison between the plasma membrane fractions and homogenates from the rat and fresh and frozen human livers. Compared with their homogenate, all the sinusoidal plasma membrane preparations were poorly enriched with Na(+)-K(+)-ATPase (2.6-8.4-fold), a sinusoidal marker enzyme… CONTINUE READING

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