Isolation of siRNA target by biotinylated siRNA reveals that human CCDC12 promotes early erythroid differentiation.

@article{Fan2012IsolationOS,
  title={Isolation of siRNA target by biotinylated siRNA reveals that human CCDC12 promotes early erythroid differentiation.},
  author={Cuiqing Fan and L. Dong and N. Zhu and Yuan Xiong and Jiewen Zhang and Le Wang and Y. Shen and Xiaoyin Zhang and Mei-hong Chen},
  journal={Leukemia research},
  year={2012},
  volume={36 6},
  pages={
          779-83
        }
}
Erythroid differentiation is a tightly regulated multi-step process that has not been fully elucidated. We previously reported that a siRNA screened from random siRNA library, siRNA clone-67, induced erythroid differentiation in human erythroleukemia K-562cell line. Here we identified that human CCDC12 (coiled-coil domain containing 12) is a target of siRNA clone-67, by target capture with biotinylated siRNA. Over-expression of CCDC12 in K-562cell up-regulated the expression of CD235, ε-globin… Expand

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