Isolation of recombinants between T7 and T3 bacteriophages and their use in vitro transcriptional mapping.

@article{Beier1977IsolationOR,
  title={Isolation of recombinants between T7 and T3 bacteriophages and their use in vitro transcriptional mapping.},
  author={H. Beier and Miriam Golomb and Michael J. Chamberlin},
  journal={Journal of virology},
  year={1977},
  volume={21 2},
  pages={753-65}
}
A variety of T3 X T7 recombinants were isolated from crosses between T3 and T7 parental phages carrying amber markers in various genes (gene 1 to gene 19). The genetic constitution of these recombinants was determined by reference to the selected markers and also directly by analysis of the proteins translated from the T3 X T7 recombinants in vivo. Although… CONTINUE READING