Isolation of proteoglycan-hyaluronate complexes from bovine aorta.

  title={Isolation of proteoglycan-hyaluronate complexes from bovine aorta.},
  author={J J McMurtrey and B. S. Bhandaru Radhakrishnamurthy and Edward R. Dalferes and Gerald Berenson and J D. Gregory},
  journal={The Journal of biological chemistry},
  volume={254 5},
Two proteoglycan-hyaluronate complexes were extracted from bovine aorta with 4.0 M guanidinium chloride in the presence of protease inhibitors and purified by cesium chloride density gradient centrifugation under associative conditions. Complex I with higher buoyant density had 22% protein and 18% uronate, whereas Complex II had 11% protein and 23.6% uronate. Glycosaminoglycan (GAG) analysis of the complexes indicated that both complexes contained large amounts of hyaluronic acid, 13.6% and 24… CONTINUE READING