Isolation of plasmid DNA from cell lysates by aqueous two-phase systems.

  title={Isolation of plasmid DNA from cell lysates by aqueous two-phase systems.},
  author={Sofia C. Ribeiro and Gabriel Ant{\'o}nio Amaro Monteiro and Joaquim Manuel Sampaio Cabral and Duarte MF Prazeres},
  journal={Biotechnology and bioengineering},
  volume={78 4},
This work presents a study of the partitioning of a plasmid vector containing the cystic fibrosis gene in polyethylene glycol (PEG)/salt (K2HPO4) aqueous two-phase systems (ATPS). The plasmid was extracted from neutralized alkaline lysates using PEG with molecular weights varying from 200 to 8000. The effects of the lysate mass loaded to the ATPS (20, 40, and 60% w/w) and of the plasmid concentration in the lysate were evaluated. The performance of the process was determined by qualitative and… CONTINUE READING

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