Isolation of pheromone synergists of bark beetle,Pityogenes chalcographus, from complex insect-plant odors by fractionation and subtractive-combination bioassay

  title={Isolation of pheromone synergists of bark beetle,Pityogenes chalcographus, from complex insect-plant odors by fractionation and subtractive-combination bioassay},
  author={John A Byers and G{\"o}ran Birgersson and Jan L{\"o}fqvist and Monica Appelgren and Gunnar Bergstr{\"o}m},
  journal={Journal of Chemical Ecology},
Capillary gas chromatography with columns of different polarity and two-dimensional fractionation of effluents were used with novel subtrac-tive-combination bioassays to rigorously isolate host- and insect-produced pheromone synergists of the bark beetlePityogenes chalcographus (Coleop-tera: Scolytidae). Methyl (E,Z)-2,4-decadienoate (E,Z-MD) and the previously identified chalcogran were found to be synergistically attractive to both sexes.E,Z-MD was produced sex-specifically in males, and only… 

Identification of Pheromone Synergists in American Palm Weevil, Rhynchophorus Palmarum, and Attraction of Related Dynamis Borassi

Thirteen host-plant kairomone blends, including 28 compounds, were tested and showed moderate to high synergy with rhynchophorol. The blends plus rhynchophorol also attracted the related Dynamis

Aggregation pheromones of bark beetles, Pityogenes quadridens and P. bidentatus, colonizing Scotch pine: olfactory avoidance of interspecific mating and competition

It is suggested that aggregation pheromone components from males of each species not only attract conspecifics but also aid individuals in avoiding interspecific mating and competition for food and spatial resources within the bark phloem layer.

Pheromone Production, Attraction, and Interspecific Inhibition among Four Species of Ips Bark Beetles in the Southeastern USA

Hindgut volatiles from attacking, unmated males of Ips avulsus, I. grandicollis, and I. pini were analyzed and a combined species-specific subtractive field bioassay was set up for the four bark beetle species to reveal strong interspecific inhibition.

Male-Produced Sex Pheromone of the Cerambycid Beetle Hedypathes betulinus: Chemical Identification and Biological Activity

The bioactivity of components of the male-produced sex pheromone of Hedypathes betulinus (Coleoptera: Cerambycidae: Lamiinae) was tested and a ternary mixture of the compounds was attractive to female beetles, although the individual compounds were not attractive by themselves.

Plant volatiles are behavioral cues for adult females of the gall wasp Antistrophus rufus

Summary.The purpose of this study was to identify plant volatiles that provide host location cues for adult females of the gall wasp Antistrophus rufus Gillette (Hymenoptera: Cynipidae). Larvae of

Lanierone: A new pheromone component fromIps pini (Coleoptera: Scolytidae) in New York

A new pheromone component, lanierone, was isolated and identified from a Porapak Q collection of volatiles from maleIps pini from New York through GC fractionation, bioassay, and spectrometry and is produced exclusively by males.

Bark Beetles, Pityogenes bidentatus, Orienting to Aggregation Pheromone Avoid Conifer Monoterpene Odors When Flying but Not When Walking

The bark beetle may avoid monoterpenes when flying as a mechanism for avoiding nonhost species, vigorous and thus unsuitable host trees, as well as harmful resinous areas of hosts.

Primary Attraction of the Fir Engraver, Scolytus ventralis

It is argued against the hypothesis of pheromone-mediated secondary attraction in S. ventralis and concluded that the attack dynamics of this species can be explained solely by its sensitive primary attraction response to host volatiles.

Electrophysiological Response of Female Dogwood Borer (Lepidoptera: Sesiidae) to Volatile Compounds from Apple Trees

Coupled gas chromatography and electroantennogram detection (GC-EAD) analyses of headspace volatiles from apple host tissues revealed a total of 16 compounds to which female dogwood borer,

Semiochemicals from bark beetles: New results, remarks, and reflections

A brief survey is given about recent results in the identification of semiochemicals in bark beetles, and the occurrence of (+)-grandisol and other compounds related to weevil pheromones points to a close relation between Scolytidae and Curculionidae.



Aggregation pheromones of the flat grain beetle,Cryptolestes pusillus (Coleoptera: Cucujidae)

Pheromone production increased dramatically when the insects were aerated on a food source and did not significantly increase the response when added to the most active mixture of I and II, and so it is probably not part of the aggregation pheromones.

Quantitative variation of pheromone components in the spruce bark beetleIps typographus from different attack phases

The correlations between the nine pheromone components and five major host monoterpenes in the gut showed that the variation in the amount of methyl-butenol, ipsenoli, and ipsdienol could not be explained by the variations in the amounts of host monoterspenes.

Differences in attraction to semiochemicals present in sympatric pine shoot beetles,Tomicus minor andT. piniperda

It is suggested that host and mate location in T. piniperda is achieved by means of a kairomone composed of host monoterpenes, whileT.

Chemical attractants for the smaller european elm bark beetleScolytus multistriatus (Coleoptera: Scolytidae)

A mixture of synthetic I and II plus natural III (from cubeb oil) was highly attractive to beetles in preliminary field tests, but a mixture of all three showed activity nearly equivalent to that of the of the original Porapak extract.

Sex pheromones and reproductive isolation in four european small ermine moths

Although these species are the two most distantly related European small ermine moths, they seem to share the same sex pheromone, i.e.,Z11–14∶OAc (+20%E) and (Z)-11-tetradecenol.


Comparative gas chromatographic analyses of volatiles generated by Ips indicate the presence of cis- and trans-verbenol in hindguts of the 12 species investigated, indicating the aggregation of aggressive bark beetle species seems to be largely dependent on contact pheromones, whereas the host’s susceptibility to feeding is indicated by the release of frass phersomones.

Attractive and inhibitory pheromones produced in the bark beetle,Dendroctonus brevicomis, during host colonization: Regulation of inter- and intraspecific competition

These inhibitory pheromones appear to cause several competing species of bark beetle to avoid landing in areas infested with D. brevicomis, even when their own phersomone is present.

Identification of volatile sex pheromone components released by the southern armyworm,Spodoptera eridania (Cramer)

The addition of (Z)-9-tetradecen-1-ol to the acetate blends tested resulted in the capture of beet armyworm,S.

Monoterpene oviposition stimulants ofDioryctria amatella in volatiles from fusiform rust galls and second-year loblolly pine cones

Volatiles collected on Poropak Q from fusiform rust galls of loblolly pine for 3 hr were better ovi-position stimulants for Dioryctria amatella (Hulst) females than extracts of 8-hr collections, and the combination of α-pinene, myrcene, and limonene was as attractive to D. amatellA females as all other terpene combinations, including turpentine.