Isolation of oxygenase genes for indigo-forming activity from an artificially polluted soil metagenome by functional screening using Pseudomonas putida strains as hosts

@article{Nagayama2014IsolationOO,
  title={Isolation of oxygenase genes for indigo-forming activity from an artificially polluted soil metagenome by functional screening using Pseudomonas putida strains as hosts},
  author={Hirofumi Nagayama and Tomonori Sugawara and Ryo Endo and Akira Ono and Hiromi Kato and Yoshiyuki Ohtsubo and Yuji Nagata and Masataka Tsuda},
  journal={Applied Microbiology and Biotechnology},
  year={2014},
  volume={99},
  pages={4453-4470}
}
Metagenomes contain the DNA from many microorganisms, both culturable and non-culturable, and are a potential resource of novel genes. In this study, a 5.2-Gb metagenomic DNA library was constructed from a soil sample (artificially polluted with four aromatic compounds, i.e., biphenyl, phenanthrene, carbazole, and 3-chlorobenzoate) in Escherichia coli by using a broad-host-range cosmid vector. The resultant library was introduced into naphthalene-degrading Pseudomonas putida-derived strains… CONTINUE READING
1
Twitter Mention

Citations

Publications citing this paper.
SHOWING 1-10 OF 14 CITATIONS

Metagenomics: novel enzymes from non-culturable microbes.

VIEW 4 EXCERPTS
CITES BACKGROUND & METHODS
HIGHLY INFLUENCED

Recent Advances in Function-based Metagenomic Screening

  • Genomics, Proteomics & Bioinformatics
  • 2018
VIEW 1 EXCERPT
CITES BACKGROUND

References

Publications referenced by this paper.
SHOWING 1-10 OF 51 REFERENCES

Molecular cloning : a laboratory manual

Joseph Sambrook, David W. Russell
  • 1983
VIEW 6 EXCERPTS
HIGHLY INFLUENTIAL

Evolutionary relationships among extradiol dioxygenases.

  • Journal of bacteriology
  • 1996
VIEW 4 EXCERPTS
HIGHLY INFLUENTIAL

Flavin dependent monooxygenases.

VIEW 4 EXCERPTS
HIGHLY INFLUENTIAL

The complete genome sequence of Cupriavidus metallidurans strain CH 34 , a master survivalist in harsh and anthropogenic environments

PJ Janssen, R Houdt, +14 authors M Mergeay
  • PLoS One
  • 2010
VIEW 1 EXCERPT
HIGHLY INFLUENTIAL