Isolation of nutrient deficient mutants and quantitative mutation assay by reversion of alanine-requiring L5178Y cells.

@article{Suzuki1976IsolationON,
  title={Isolation of nutrient deficient mutants and quantitative mutation assay by reversion of alanine-requiring L5178Y cells.},
  author={Nobuo N. Suzuki and Shoji Okada},
  journal={Mutation research},
  year={1976},
  volume={34 3},
  pages={489-505}
}
Nutrient deficient cells for alanine or purine were isolated from L5178Y cells by the BudR-light method of Puck and Kao. The purine requiring cells, named P, showed maximum-plating efficiency in the presence of 10(-3)-10(-4) M inosine. The optimum concentration of L-alpha-alanine for Ala 32, one of alanine requiring mutants, was 10(-3) M. When Ala 32 cells were depleted of alanine, they showed an immediate decrease in incorporation of protein and DNA precursors without much change in that of… CONTINUE READING