Isolation of novel Pseudomonas syringae promoters and functional characterization in polyhydroxyalkanoate-producing pseudomonads.

@article{Solaiman2010IsolationON,
  title={Isolation of novel Pseudomonas syringae promoters and functional characterization in polyhydroxyalkanoate-producing pseudomonads.},
  author={Daniel K. Y. Solaiman and Bryan M. Swingle},
  journal={New biotechnology},
  year={2010},
  volume={27 1},
  pages={1-9}
}
A library of genomic DNA fragments of Pseudomonas syringae pv. tomato DC3000 was constructed in a lacZalpha-containing plasmid, pBS29. The library was used in a preliminary alpha-complementation-based screen to identify clones with promoter activity in Escherichia coli. Ten positive clones were sequenced and their locations in the chromosomal DNA of DC3000 strain were mapped. Five positive clones (P2, P3, P4, P6 and P8) were further assayed for promoter activity in three polyhydroxyalkanoate… CONTINUE READING

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