Isolation of nonsense mutants of lipid-containing bacteriophage PRD1.

  title={Isolation of nonsense mutants of lipid-containing bacteriophage PRD1.},
  author={Leonard Mindich and D. G. Bamford and Claire D. Goldthwaite and Maureen Laverty and Grahame Mackenzie},
  journal={Journal of virology},
  volume={44 3},
We isolated nonsense mutants of bacteriophage PRD1, a lipid-containing polyhedral virus capable of infecting many genera of gram-negative bacteria. These mutants were grouped into 19 classes on the basis of genetic complementation and sodium dodecyl sulfate-polyacrylamide gel electrophoretic analysis. PRD1 infection led to the synthesis of at least 25 viral proteins, 17 of which were components of mature virions. The synthesis of proteins fell into the following three classes: very early… CONTINUE READING

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