Isolation of nonhistone-protein-rich chromatin fragments differing in composition and recovery between transcriptionally active and inactive sources.


Soluble chromatin was fragmented by deoxyribonuclease I[EC] or II[EC] digestion or sonication, followed by 0.7 m NaCl treatment, which preferentially removed DNA fragments and histones from the fragmented chromatin, but not from intact chromatin, leaving an insoluble fraction of DNA-histone-nonhistone protein complex without appreciable… (More)


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