Isolation of new ribozymes from a large pool of random sequences [see comment].

@article{Bartel1993IsolationON,
  title={Isolation of new ribozymes from a large pool of random sequences [see comment].},
  author={David P. Bartel and Jack W. Szostak},
  journal={Science},
  year={1993},
  volume={261 5127},
  pages={
          1411-8
        }
}
An iterative in vitro selection procedure was used to isolate a new class of catalytic RNAs (ribozymes) from a large pool of random-sequence RNA molecules. These ribozymes ligate two RNA molecules that are aligned on a template by catalyzing the attack of a 3'-hydroxyl on an adjacent 5'-triphosphate--a reaction similar to that employed by the familiar protein enzymes that synthesize RNA. The corresponding uncatalyzed reaction also yields a 3',5'-phosphodiester bond. In vitro evolution of the… 

A ribozyme that ligates RNA to protein

  • S. BaskervilleD. Bartel
  • Biology
    Proceedings of the National Academy of Sciences of the United States of America
  • 2002
An optimized version of the ribozyme reacts with substrate-fusion proteins, allowing it to be used to attach RNA tags to proteins both in vitro and within bacterial cells, suggesting a simple way to tag a specific protein with amplifiable information.

In vitro evolution of new ribozymes with polynucleotide kinase activity

An engineered version of one class of kinases is able to catalyse the transfer of thiophosphate from ATP-γS to the 5′-hydroxyl of an exogenous oligoribonucleotide substrate with multiple turnover, thus acting as a true enzyme.

Creation and evolution of new ribozymes.

An RNA is generated that synthesizes RNA using the same reaction as that employed by protein enzymes that catalyze RNA polymerization, and shows significant template fidelity.

In vitro evolution of a self-alkylatlng ribozyme

RNA enzymes are postulated to have catalysed all chemical reactions in the earliest living cells, and selection for self-biotinylation yields a transfer RNA-like ribozyme that efficiently catalyses carbon–nitrogen bond formation.

The New World of ribozymes.

  • L. Jaeger
  • Biology
    Current opinion in structural biology
  • 1997

Ribozyme-Catalyzed Transcription of an Active Ribozyme

The evolution and engineering of an RNA polymerase ribozyme capable of synthesizing RNAs of up to 95 nucleotides in length is described, and the accurate synthesis of an enzymatically active RNA, a hammerhead endonuclease ribo enzyme is demonstrated.

Ribozyme-catalysed amino-acid transfer reactions

In vitro selection and evolution is used to isolate ribozymes with acyl transferase activity from a pool of random RNA sequences and one of them transfers an amino acid to itself in a reaction that is analogous to peptidyl transfer on the ribosome.

New ligase-derived RNA polymerase ribozymes.

Eight ligase-derived polymerase ribozymes isolated from a pool of variants of an existing RNA ligase ribozyme are reported, each a new potential starting point for further in vitro evolution and engineering and together substantially enrich the set of candidates from which an RNA replicaseribozyme might eventually emerge.

Conversion of a ribozyme to a deoxyribozyme through in vitro evolution.

...

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