Isolation of neutralizing anti-C5a monoclonal antibodies from a filamentous phage monovalent Fab display library.

@article{Ames1994IsolationON,
  title={Isolation of neutralizing anti-C5a monoclonal antibodies from a filamentous phage monovalent Fab display library.},
  author={Robert S. Ames and Mark A. Tornetta and Catherine S. Jones and Ping Tsui},
  journal={Journal of immunology},
  year={1994},
  volume={152 9},
  pages={4572-81}
}
A panel of mAbs against the activated complement component C5a was obtained from a filamentous phage M13-Fab display library generated from mice immunized with human rC5a. Fabs isolated from the library after iterative selection vs rC5a bound to both rC5a and purified C5. To isolate Fabs specific for neoepitopes expressed on C5a but not on the native… CONTINUE READING