Isolation of neuronal parvalbumin by high-performance liquid chromatography. Characterization and comparison with muscle parvalbumin.

@article{Berchtold1982IsolationON,
  title={Isolation of neuronal parvalbumin by high-performance liquid chromatography. Characterization and comparison with muscle parvalbumin.},
  author={Martin W Berchtold and Kenneth J Wilson and Claus Wilhelm Heizmann},
  journal={Biochemistry},
  year={1982},
  volume={21 25},
  pages={6552-7}
}
Neuronal parvalbumin has been isolated from rat brain and purified to homogeneity by high-performance liquid chromatography (HPLC) on reverse-phase supports. This procedure includes four consecutive chromatographic steps with an overall protein recovery of 74% and a 26 400-fold purification. The concentration of parvalbumin was found to be approximately 10 mg/kg wet weight in brain tissue, which is about 100 times lower than that in rat muscle. The physical properties of brain parvalbumin are… CONTINUE READING