Isolation of mutants of an animal virus in bacteria.

@article{Peden1980IsolationOM,
  title={Isolation of mutants of an animal virus in bacteria.},
  author={Keith W. C. Peden and James M. Pipas and Sonia Pearson-White and Daniel Nathans},
  journal={Science},
  year={1980},
  volume={209 4463},
  pages={1392-6}
}
Mutants of animal viruses can be isolated in bacteria by recombinant DNA methods. Since no viral functions are required for propagation of recombinants in bacteria, viral mutants with lethal changes in cis- or trans-acting elements can be isolated, as well as partially or conditionally defective mutants. In the cases of viruses with small DNA genomes, such as the tumorigenic simian virus 40 (SV40), the entire viral DNA can be inserted into the bacterial plasmid pBR322 and cloned in Escherichia… CONTINUE READING

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