Isolation of mutants of Saccharomyces cerevisiae requiring DNA topoisomerase I.

@article{Sadoff1995IsolationOM,
  title={Isolation of mutants of Saccharomyces cerevisiae requiring DNA topoisomerase I.},
  author={B U Sadoff and Sharon Heath-Pagliuso and Irene B Casta{\~n}o and Yichao Zhu and F. Scott Kieff and Michael F. Christman},
  journal={Genetics},
  year={1995},
  volume={141 2},
  pages={465-79}
}
Despite evidence that DNA topoisomerase I is required to relieve torsional stress during DNA replication and transcription, yeast strains with a top1 null mutation are viable and display no gross defects in DNA or RNA synthesis, possibly because other proteins provide overlapping functions. We isolated mutants whose inviablility or growth defect is relieved when TOP1 is expressed [trf mutants (topoisomerase one-requiring function)]. The TRF genes define at least four complementation groups… CONTINUE READING

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