Isolation of murine aortic endothelial cells in culture and the effects of sex steroids on their growth

  title={Isolation of murine aortic endothelial cells in culture and the effects of sex steroids on their growth},
  author={David W. Lincoln and Ann M. Larsen and Patricia G. Phillips and Kathleen Bove},
  journal={In Vitro Cellular & Developmental Biology - Animal},
The lack of commercially available primary murine endothelial cells prompted us to isolate and cultivate this cell type. We report here the effect of sex steroids on the in vitro growth of murine aortic endothelial cells. Murine aortic endothelial cells were isolated by a combination of explant outgrowth from aortic rings and enzymatic digestion. The endothelial nature of the cells was verified by uptake of acylated low-density lipoprotein and positive staining for CD-31. Murine aortic… CONTINUE READING


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