Isolation of monoclonal antibodies to phencyclidine from ascites fluid by preparative isoelectric focusing in the Rotofor.

Abstract

A monoclonal antibody to phencyclidine was developed, produced in mouse ascites fluid, and purified. The purification used only preparative-scale isoelectric focusing in the Rotofor and dialysis. In 4 h, 25% (4 mg) of the antibody from 10 ml of ascites fluid was purified to homogeneity while 63% of the total antibody was recovered.

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@article{Egen1988IsolationOM, title={Isolation of monoclonal antibodies to phencyclidine from ascites fluid by preparative isoelectric focusing in the Rotofor.}, author={Nathalie Egen and Melanie Bliss and Michael Mayersohn and S Michael Owens and Larry W. Arnold and Monika Bier}, journal={Analytical biochemistry}, year={1988}, volume={172 2}, pages={488-94} }