Isolation of monoclonal antibodies that recognize the transforming proteins of avian sarcoma viruses.

@article{Lipsich1983IsolationOM,
  title={Isolation of monoclonal antibodies that recognize the transforming proteins of avian sarcoma viruses.},
  author={Leah Ann Lipsich and A. J. Lewis and Joan S. Brugge},
  journal={Journal of virology},
  year={1983},
  volume={48 2},
  pages={
          352-60
        }
}
Thirteen clones of hybrid cells which synthesize antibodies directed against the Rous sarcoma virus (RSV) transforming protein, pp60src, were isolated. Mouse myeloma cells were fused with spleen cells from mice that had been immunized with purified pp60src from bacterial recombinants which direct the synthesis of the RSV src gene. The hybridomas which survived the selection medium were screened by immunoprecipitation of pp60src from 32P-labeled lysates of RSV-transformed cells. Monoclonal… CONTINUE READING

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