Isolation of mitochondria from procyclic Trypanosoma brucei.

@article{Schneider2007IsolationOM,
  title={Isolation of mitochondria from procyclic Trypanosoma brucei.},
  author={A. Schneider and Fabien Charrière and M. Pusnik and E. Horn},
  journal={Methods in molecular biology},
  year={2007},
  volume={372},
  pages={
          67-80
        }
}
The mitochondrion of the parasitic protozoon Trypanosoma brucei shows a number of unique features, many of which represent highly interesting research topics. [...] Key Method In the first protocol, the cells are lysed under hypotonic conditions, and mitoplast vesicles are isolated on Percoll gradients; in the second method, lysis occurs isotonically by N2 cavitation, and the mitochondrial vesicles are isolated by Nycodenz gradient centrifugation.Expand
A Scalable Purification Method for Mitochondria from Trypanosoma brucei.
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ATP regulates the activity of an alternative oxidase in Trypanosoma brucei
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Divergent Small Tim Homologues Are Associated with TbTim17 and Critical for the Biogenesis of TbTim17 Protein Complexes in Trypanosoma brucei
TLDR
This study characterized three T. brucei small Tim proteins and found that these three small TbTims are expressed as soluble mitochondrial intermembrane space proteins that aid in the import of mitochondrial inner membrane proteins with internal targeting signals. Expand
A component of the mitochondrial outer membrane proteome of T. brucei probably contains covalent bound fatty acids.
A subclass of eukaryotic proteins is subject to modification with fatty acids, the most common of which are palmitic and myristic acid. Protein acylation allows association with cellular membranes inExpand
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