Isolation of mature spinal motor neurons and single-cell analysis using the comet assay of early low-level DNA damage induced in vitro and in vivo.

@article{Liu2001IsolationOM,
  title={Isolation of mature spinal motor neurons and single-cell analysis using the comet assay of early low-level DNA damage induced in vitro and in vivo.},
  author={Zhiping Liu and Lee J. Martin},
  journal={The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society},
  year={2001},
  volume={49 8},
  pages={957-72}
}
We developed an isolation technique for motor neurons from adult rat spinal cord. Spinal cord enlargements were discretely microdissected into ventral horn tissue columns that were trypsin-digested and subjected to differential low-speed centrifugation to fractionate ventral horn cell types. A fraction enriched in alpha-motor neurons was isolated. Motor neuron enrichment was verified by immunofluorescence for choline acetyltransferase and prelabeling axon projections to skeletal muscle. Adult… CONTINUE READING
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