Isolation of matrix protein M1 from influenza viruses by acid-dependent extraction with nonionic detergent.

@article{Zhirnov1992IsolationOM,
  title={Isolation of matrix protein M1 from influenza viruses by acid-dependent extraction with nonionic detergent.},
  author={Oleg P. Zhirnov},
  journal={Virology},
  year={1992},
  volume={186 1},
  pages={324-30}
}
Influenza viruses were disrupted layer by layer with the nonionic detergent NP-40 at fixed pH. Treatment of the virions with NP-40 at neutral or mildly alkaline pH (6.8-8.0) yielded viral core structures containing M1 protein. The matrix M1 protein was selectively extracted from cores at acidic pH 3.0-4.5 with citrate, acetate, and phosphate buffers or with morpholinoethanesulfonic acid. The resulting M1 protein sedimented in a glycerol gradient with a coefficient of 2.8 S and most likely… CONTINUE READING

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