Isolation of large numbers of highly purified lymphocytes and monocytes with a modified centrifugal elutriation technique.

@article{Figdor1981IsolationOL,
  title={Isolation of large numbers of highly purified lymphocytes and monocytes with a modified centrifugal elutriation technique.},
  author={C G Figdor and Willy S. Bont and Jan de Vries and W L van Es},
  journal={Journal of immunological methods},
  year={1981},
  volume={40 3},
  pages={
          275-88
        }
}
A modified centrifugal elutriation technique is described for the isolation of large numbers of lymphocytes and monocytes. Elutriation was carried out by lowering the rotor speed at a constant flow rate which was generated by hydrostatic pressure. The flow rate could be kept constant if the separation procedure was performed at high pressure and high systemic resistance. Up to 2.3 X 10(9) mononuclear cells derived from 2000 ml blood were separated in one single experiment in approximately 1 h… CONTINUE READING

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