Isolation of large bacterial plasmids and characterization of the P2 incompatibility group plasmids pMG1 and pMG5.

@article{Hansen1978IsolationOL,
  title={Isolation of large bacterial plasmids and characterization of the P2 incompatibility group plasmids pMG1 and pMG5.},
  author={J B Hansen and Ronald H. Olsen},
  journal={Journal of bacteriology},
  year={1978},
  volume={135 1},
  pages={227-38}
}
Large plasmids from Agrobacterium tumefaciens, Salmonella typhimurium, Escherichia coli, Pseudomonas putida, and Pseudomonas aeruginosa were routinely and consistently isolated using a procedure which does not require ultracentrifugation but includes steps designed to separate large-plasmid DNA from the bacterial folded chromosome. It also selectively removes fragments of broken chromosome. A variety of large plasmids was readily visualized with agarose gel electorphoresis, including five… CONTINUE READING

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