Isolation of isomangiferin from honeybush (Cyclopia subternata) using high-speed counter-current chromatography and high-performance liquid chromatography.

@article{Beer2009IsolationOI,
  title={Isolation of isomangiferin from honeybush (Cyclopia subternata) using high-speed counter-current chromatography and high-performance liquid chromatography.},
  author={Dalene de Beer and Gerold Jerz and Elizabeth Joubert and Victor Wray and Peter 7 Winterhalter},
  journal={Journal of chromatography. A},
  year={2009},
  volume={1216 19},
  pages={4282-9}
}
Isomangiferin was isolated from Cyclopia subternata using a multi-step process including extraction, liquid-liquid partitioning, high-speed counter-current chromatography (HSCCC) and semi-preparative reversed-phase high-performance liquid chromatography (HPLC). Enrichment of phenolic compounds in a methanol extract of C. subternata leaves was conducted using liquid-liquid partitioning with ethyl acetate-methanol-water (1:1:2, v/v). The enriched fraction was further fractionated using HSCCC with… CONTINUE READING

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