Isolation of intact nuclei from hematopoietic cell types.

  title={Isolation of intact nuclei from hematopoietic cell types.},
  author={Toni M. Antalis and D Godbolt},
  journal={Nucleic acids research},
  volume={19 15},
Isolation of nuclei is widely used in analyses such as DNA footprinting, gel retardation and runon transcription assays. When we applied the NP-40 lysis method of Nevins (1) to progenitors of the monocytic lineage, we found that these cells have a very fragile nuclear membrane which is easily susceptible to partial premature lysis. Therefore, we developed a modified protocol to allow rapid isolation of intact nuclei in 95-100% yield. The human hematopoietic cells, such as HL-60 and U937, are… CONTINUE READING


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R Nevinsj

Nucl. Acids Res

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