Isolation of intact elastin fibers devoid of microfibrils.

  title={Isolation of intact elastin fibers devoid of microfibrils.},
  author={Willeke F Daamen and Theo G. Hafmans and Jacques H. Veerkamp and Toin H. van Kuppevelt},
  journal={Tissue engineering},
  volume={11 7-8},
Purification protocols for elastin generally result in greatly damaged elastin fibers and this likely influences the biological response. We here describe a novel protocol for the isolation of elastin whereby the fibers stay intact, and introduce the term "elastin fiber" for intact elastic fibers with elastin as their sole component. As opposed to elastic fibers, elastin fibers do not contain any microfibrils or associated molecules. Elastin fibers were isolated from equine elastic ligaments… CONTINUE READING