Isolation of immunoreactive components from experimental and human tumour tissues and serums by high-performance gel chromatography.

A method for the rapid separation of proteinous fractions by high-performance gel chromatography was described. Homogenates from tumorous and healthy tissues and blood were eluted by saline on a column packed with rigid hydrophilic macroporous particles of O-glucose--Spheron 300. Fractions were collected and subjected to further analyses. Their antigenic… (More)