Isolation of immediate-early differentiation mRNAs by enzymatic amplification of subtracted cDNA from human endothelial cells.

@article{Hla1990IsolationOI,
  title={Isolation of immediate-early differentiation mRNAs by enzymatic amplification of subtracted cDNA from human endothelial cells.},
  author={Timothy Hla and Thomas Maciag},
  journal={Biochemical and biophysical research communications},
  year={1990},
  volume={167 2},
  pages={
          637-43
        }
}
The phenomena of cell growth and differentiation result from unique transcriptional programs. Inducible genes are therefore differentially expressed in response to various biochemical stimuli that regulate cell division and differentiation. To isolate differentially expressed mRNAs of unknown sequence, two techniques, differential and subtractive hybridization, have been used. Subtractive hybridization is superior for the isolation of rare mRNAs; however, its utility is somewhat limited by the… CONTINUE READING
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