Isolation of human NK cells and generation of LAK activity.

@article{Whiteside2001IsolationOH,
  title={Isolation of human NK cells and generation of LAK activity.},
  author={Theresa L Whiteside},
  journal={Current protocols in immunology},
  year={2001},
  volume={Chapter 7},
  pages={Unit 7.7}
}
This unit describes the preparation of natural killer (NK) cells from normal human peripheral blood and their incubation in vitro in the presence of interleukin 2 (IL-2) to yield lymphokine-activated killer (LAK) cells. A protocol is presented for isolating highly purified NK cell populations from PBMC, and another method presents steps for generating LAK cells from these purified NK cells via IL-2 stimulation. An alternate protocol describes the generation of LAK cells directly from whole… CONTINUE READING